Anti Type III Collagen
Mouse monoclonal antibody
Cat.No. CSI 007-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.
Acid-digested pepsin soluble human type III collagen
CSI 007-01 is highly specific for type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
CSI 007-01 shows reactivity with human, dog, rat and kangaroo type III collagens by ELISA and immunoblotting. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and immunoblotting.
Epitope is located approximately 100nm from the N-terminal of the molecule
CSI 007-01 can be used for detection of collagens by ELISA. CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human (3) and kangaroo (4) skin, and of new dog tissue associated with biomaterial implants (2,5). If fixation of tissue is required, acetone or ethanol is recommended.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:800 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Yes | 2, 3, 4, 5 |
1. Werkmeister JA, Ramshaw JAM (1991) Multiple antigenic determinants on type III collagen. Biochem J 274:895-898.
2. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
3. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
4. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.
5. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119.
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CSI 007-01.pdf (24.91 kB) Product specification
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April 23 2008
